Fluorescence Microscopy allows highly specific imaging of cellular compartments in a minimal invasive fashion while obtaining the highest contrast. However, conventional microscopes are limited in their resolution due to the diffraction barrier. In the past decades several different approaches have shown the ability to circumvent the diffraction limit by either switching on and off single fluorophores, utilizing structured illumination, optical fluctuation or alternatively depleting excited chromophores from the excitation volume. This document provides a quick overview of the STED technique introduced by Stefan W. Hell in 1994. NKT Photonics’ SuperK and Onefive KATANA HP lasers are perfect combination to realize flexible pulsed excitation as well as synchronized depletion within the visible and near infrared range. Read more
Laser cooling and trapping is the ability to cool atoms down to unprecedented kinetic temperatures, and to confine and support isolated atoms in “atom traps”. This unique new level of control of atomic motion allows researchers to study the behavior of atoms and quantum mechanical properties.
Different atomic cooling techniques exist, e.g. Doppler cooling, Sub–Doppler technique (used when the Doppler limit is reached - natural linewidth of the atomic transition), and atom evaporation in a Bose-Einstein condensate to slow the atom further.
Typical applications include time/frequency standards (atomic clocks), GPS systems and navigation, research on fundamental constants, quantum information (computing and encryption), and atom interferometry (gravitational detection of natural resources, potentially oil & gas). Read more
Solar cells can be characterized using a SuperK supercontinuum laser as a sun simulator, either with bulky samples, or to look at microscale features such as plasmonic particles for up- or down-conversion mechanisms; this often requires broadband excitation with a spectral shape as close as possible to the sun spectrum.
Measurement of the absorption/transmission properties of an optical fiber or a connector, or the dispersion of an optical fiber. The most prevaling techniques used for fiber characterization using supercontinuum sources are absorption spectroscopy, SNOM/NSOM and white light inteferometry. Read more
Characterization of nanoparticles - particles between 1 and 100 nanometers in size. We are often linked to localization of light in nanosized particles or interfaces that typically show a huge field enhancement at their surfaces. Typical examples are tiny single photonic components such as complex nano-waveguides, nano-apertures and nano-resonators. Tight localization is of great practical importance, e.g. for high resolution inspection, local modification of materials, high field concentration, intensity enhancement, increase of efficiency of nonlinear processes such as Raman scattering and harmonic generation Read more
Preclinical imaging is the visualization of living animals for research purposes, such as drug development. Imaging modalities have long been crucial to the researcher in observing changes, either at the organ, tissue, cell, or molecular level, in animals responding to physiological or environmental changes. Read more